After the video ends, the file deletes itself, but not before corrupting every other image file on the user's computer, replacing them with a single frame from the video.

In the mythos, 24644.mp4 is more than just a scary movie; it is described as a "sentient" file:

At the 6:44 mark, the figure stops moving and stares directly into the camera. Those who claim to have seen it say the video then displays personal information about the viewer—their current address, heartbeat rate, or even the date of their death. 💻 Technical Anomalies